We report a cell-free system that measures transport-coupled maturation of carboxypeptidase Y (CPY). the principal CPY processing enzyme proteinase A) and acceptor membranes from a yeast strain indicating intercompartmental transfer. Cytosol made from a yeast strain deleted for the gene was less efficient at driving transport. Moreover antibodies against Vps33p (a Sec1 homologue) and Vam3p… Continue reading We report a cell-free system that measures transport-coupled maturation of carboxypeptidase