d-Alanine:d-alanine ligase (EC 6. significant variations in affinity, using the 1st binding site exhibiting high affinity. DCS inhibits the enzyme, having a 50% inhibitory focus (IC50) of 0.37 mM under standard assay circumstances, implicating a preferential and weak inhibition at the next, lower-affinity binding site. Furthermore, DCS binding can be tighter at higher ATP concentrations.… Continue reading d-Alanine:d-alanine ligase (EC 6. significant variations in affinity, using the 1st