Background The formation of two distinctive cell lineages in preimplantation mouse embryos is characterized by differential gene expression. We constructed a duplex RT-LATE-PCR assay for real-time measurement of Oct4 and Xist templates and confirmed its specificity and quantitative accuracy with different methods. We then undertook analysis of sets of blastomeres isolated from embryos at the… Continue reading Background The formation of two distinctive cell lineages in preimplantation mouse