Mutations in cobalamin or B12 trafficking genes needed for cofactor assimilation and targeting result in inborn mistakes of cobalamin rate of metabolism. was exchanged with 100 mM Hepes pH 7.0 150 mM KCl 10 glycerol (Buffer B). 1.4 Size exclusion chromatography The oligomeric condition from the CblD variations was dependant on launching 1-2.5 mg protein… Continue reading Mutations in cobalamin or B12 trafficking genes needed for cofactor assimilation