Supplementary MaterialsSupplementary Fig. whole wheat, peanut, walnut, crab, shrimp, apple, and peach). Results PROTIA? Specific IgG4? showed 95.6% agreement rate with ImmunoCAP? in the positivity assessment. For sIgG4 positivity to each individual allergen, an agreement rate of more than 84.8% was observed. In Cohen’s kappa analysis, these assays displayed considerable correlations [Cohen’s kappa coefficient () 0.699], except for shrimp (=0.448). Furthermore, both assays displayed strong correlations in quantitative comparisons [correlation coefficients value () 0.8014], except for apple (=0.6571, spores32Buckwheat12spores33Rsnow13spores34Soy bean14Bee venom35Peanut15Wasp venom36Walnut16Cockroach37Almond17Pork38Peach18Beef39Apple19Chicken40Kiwi20Egg yolk41Mango21Egg white42-Gal Open in a separate window MATERIALS AND METHODS Study participants Five hundred and nineteen Korean allergy individuals who visited the Allergy and Asthma Center of Severance Hospital for the analysis and treatment of their allergic diseases from August 2013 to May 2019 were enrolled in this study. The demographic characteristics of the individuals are summarized in Table 2. There were 310 males and 209 females. The mean age of the individuals was 24.016.9 years (meanstandard deviation). Three hundred and eighteen individuals (61.3 %) had allergic rhinitis, 198 (38.2%) individuals had atopic dermatitis, 146 individuals (28.1%) had food allergy, and 114 individuals (22.0%) had asthma. Individuals did not possess other chronic diseases, including autoimmune diseases, cancer, chronic infections, or additional immune-related diseases. Blood samples were collected from all individuals to perform the two diagnostic checks, PROTIA? Specific IgG4? and ImmunoCAP?. The total number of combined checks was 731, and the most frequently tested allergen was (235), followed by (176), spores (36), walnut (33), casein (31), cat dander (30), puppy dander (26), egg white (24), wheat (24), peanut (23), peach (17), crab (16), shrimp (16), apple (15), birch pollen (10), oak pollen (7), ragweed pollen (7), and mugwort pollen (5). The Severance Hospital Ethical Review Table approved this study (No. 4-2017-1258), and knowledgeable consent was from all 519 individuals. Table 2 Demographic Characteristics of the 519 Analyzed Subjects in Accordance with the Allergens spores3625:1117.014.336 (100)0 (0)0 (0)22 (61.1)14 (38.9)7 (19.4)9 (25.0)Egg IL1F2 white2417:074.83.824 (100)0 (0)0 (0)3 (12.5)3 (12.5)12 (50.0)21 (87.5)Casein3119:129.313.324 (77.4)7 (22.6)0 (0)2 (6.5)5 (16.1)18 (58.1)28 (90.3)Wheat2416:0812.816.818 (75.0)6 (25.0)0 (0)3 (12.5)0 (0)8 (33.3)14 (58.3)Peanut2312:117.59.220 (87.0)2 (8.7)1 (4.3)3 (13.0)3 (13.0)9 (39.1)15 (65.2)Walnut3317:168.44.932 (97.0)1 (3.0)0 (0)5 (14.7)4 (11.8)10 (29.4)15 (44.1)Crab169:0743.417.214 (87.5)2 (12.5)0 (0)2 (12.5)0 (0)1 (6.3)10 (62.5)Shrimp1611:0531.921.514 (87.5)2 (12.5)0 (0)5 (31.3)1 (6.3)1 (6.3)13 (81.3)Apple159:0631.920.615 (100)0 (0)0 (0)10 (66.7)2 (13.3)2 (13.3)12 SVT-40776 (Tarafenacin) (80.0)Peach178:0923.214.914 (82.4)2 (11.8)1 (5.9)4 (23.5)1 (5.9)4 (23.5)5 (29.4)Total731 (519 individuals)310:20924.016.9665 (90.8)38 (5.2)28 (3.8)318 (61.3)114 (22.0)198 (38.2)146 (28.1) Open in a separate screen AR, allergic rhinitis; SVT-40776 (Tarafenacin) Advertisement, atopic dermatitis. Data are provided as amount (%) unless usually noticed. Serum planning and allergen selection Five milliliters of entire blood was gathered in vacuum pressure pipe (Vacuette?; Greiner Bio-One GmbH, Kremsmnster, Austria) for serum parting via centrifugation at 3000 rpm for 5 min, and the supernatant was aliquoted into several round-bottom tubes (5-mL BD Falcon? tubes; BD Bioscience Finding Labware, Bedford, MA, USA), and stored at ?76 until further use. Eighteen culprit allergens were selected for measuring sIgG4: nine inhalant allergens (spores) and nine food allergens (egg white, casein, wheat, peanut, walnut, crab, shrimp, apple, and peach). In vitro sIgG4 measurements Both PROTIA? Specific IgG4? and ImmunoCAP? sIgG4 assays were performed according to the manufacturers’ instructions. The researcher carrying out the PROTIA? Specific IgG4? assay was blinded to the results of ImmunoCAP?. The sIgG4 detection range SVT-40776 (Tarafenacin) of PROTIA? Specific IgG4? was 0.50C30 mg/L, and that of ImmunoCAP? was 0.07C30 mg/L. To compare the agreement rate of these two assays, sIgG4 ideals 0.50 mg/L were regarded as positive. Serial dilution test sIgG4 levels were measured by PROTIA? Specific IgG4? using serial dilutions of patient sera. Sera with high IgG4 titers against spore135135100.01?Egg white12121212100.01?Casein211022996.80.924?Wheat159159100.01?Peanut71691491.30.810?Walnut1221171684.80.699?Crab016016100.0N/AShrimp11531387.50.448*Apple697893.30.865?Peach11610794.10.876?Total29943231941295.60.910? Open in a separate window N/A, not relevant. =Cohen’s kappa coefficient, ideals were classified as almost perfect (0.8C1.0), substantial (0.6C0.8), moderate (0.4C0.6), fair (0.2C0.4), or poor correlations ( 0.2). *spores, shrimp, and apple) are demonstrated in Supplementary Fig. 1 (only online). The ideals of the two assays showed consistent tendencies, and.