Supplementary Materialsmolecules-25-02372-s001. exerting powerful anti-inflammatory [8,9] and cytotoxic activities [10,11] have also been identified in several members of the family. Such constituents are scarcely distributed in the herb kingdom and have mainly been reported in the Orchidaceae  and the Combretacae families [12,13]. Regarding Juncaceae, a lot of the chemical substance studies centered on the genus resulting in the identification greater than 100 phenanthrenoids . If a big selection of phenanthrenoids have already been referred to Also, many of them have a very phenanthrene or a 9,10-dihydrophenanthrene backbone, substituted at positions 2 frequently, 5 and 7 with hydroxyl, methyl, or vinyl fabric groups. A vinyl fabric group at placement 5 in the phenanthrene skeleton could possibly be regarded as a chemotaxonomic marker for types . To your knowledge, not a lot of investigations have already been reported in the types specifically on (appears to include phenanthrenoids [1,15]. This course of substances may different have natural properties such as for example antitumor, anxiolytic, antimicrobial, anti-inflammatory and spasmolytic activities . Considering the solid chemotaxonomical and natural passions of phenanthrenoids, today’s study aimed to research the chemical substance composition of the methanolic remove from aerial parts concentrating particularly PSI-7977 supplier upon this chemical substance class. Furthermore, antiproliferative and anti-inflammatory properties from the studied extract and its own isolated materials were evaluated. 2. Dialogue and Outcomes A methanolic remove, prepared through the aerial elements of was partitioned using increasing polarity liquid-liquid extraction. The five fractions (( 0.05 compared with Control. All results are expressed as a percentage, with control (i.e., cells with PMA but without extract) normalized as 100%. Further purifications were performed around PSI-7977 supplier the 247.1127 (calcd 247.1128) corresponding to a molecular formula of C18H16O. The 1H NMR data showed the presence of three vinylic protons, six aromatic methine protons, two methyl groups and a hydroxyl group. A comparison of the NMR data of compound 4 with those obtained for juncunol and the HRESIMS data, suggested the presence of a phenanthrene backbone. The 13C NMR data confirm the presence of the vinylic function (265.1242 corresponding to a molecular formula of C18H18O2 (calculated 265.1234). Comparison with the 1H and the 13C NMR spectra of compound 3 suggested that compound 5 possesses ICAM3 a 9,10-dihydrophenanthrene backbone with a vinyl group located PSI-7977 supplier at C-5, a methyl group at C-7 and a hydroxyl group at C-2. The 1H and 13C data established the presence of a hydroxylmethyl group (263.1426, [M + H ? H2O]+; calculated 263.1430) as well as the presence of a hydroxyl group. The chemical shift of a methine at 251.1077 (calcd 251.1078) corresponding to a molecular formula of C17H16O2. As the 1H and13C NMR data were PSI-7977 supplier strongly similar to compound 3 NMR data, we could identify compound 9 as a 9,10-dihydrophenanthrene backbone possessing three substituents, two methyl groups at C-1 and C-7, and a hydroxyl group at C-2. The 1H and 13C NMR data showed the presence an aldehyde function with the corresponding carbon at 0.05 compared with Control. All results are expressed as a percentage, with control (i.e., cells with PMA but without extract) normalized as 100%. In addition to their anti-inflammatory activity, phenanthrenes were known to exhibit promising in vitro antiproliferative activities on various malignancy cell lines [9,31,32]. Thus, the cytotoxicity of the isolated compounds was evaluated with a resazurin assay on THP-1, a monocytic leukemia cell line. Table 1 summarized the IC50 values obtained for each compound. With exception of compounds 1 and 9, all components revealed a strong cytotoxic activity, with IC50 below 15 M. The most effective compounds.