Supplementary MaterialsFigure 1source data 1: Quantitative characterization of clone morphology in wild-type tissues. department orientation in wild-type tissue. elife-23279-fig2-figsupp1-data1.xlsx (15K) DOI:?10.7554/eLife.23279.016 Figure 3source data 1: Quantitative analysis of sister cell contact in Fzd7-PDB expressing tissues. elife-23279-fig3-data1.xlsx (24K) DOI:?10.7554/eLife.23279.022 Body 3source data 2: Quantitative evaluation of cell pivot Hycamtin kinase activity assay in Fzd7-PDB expressing tissue. elife-23279-fig3-data2.xlsx (18K) DOI:?10.7554/eLife.23279.023 Body 3source data 3: Quantitative analysis of sister cell contact in DVL2-PDZ expressing tissue. elife-23279-fig3-data3.xlsx (24K) DOI:?10.7554/eLife.23279.024 Body 3source data 4: Quantitative analysis of cell pivot in DVL2-PDZ expressing tissue. elife-23279-fig3-data4.xlsx (17K) DOI:?10.7554/eLife.23279.025 Body 3source data 5: Quantitative analysis of sister cell contact in Fzd7 expressing tissues. elife-23279-fig3-data5.xlsx (23K) DOI:?10.7554/eLife.23279.026 Body 3source data 6: Quantitative analysis of sister cell contact in Vangl2 expressing tissue. elife-23279-fig3-data6.xlsx (24K) DOI:?10.7554/eLife.23279.027 Body 3figure health supplement 1source data 1: Characterizing cell orientation in wild-type, Fzd7, Vangl2 or Fzd7-PDB expressing tissue. elife-23279-fig3-figsupp1-data1.xlsx (12K) DOI:?10.7554/eLife.23279.020 Body 3figure health supplement 2source data 1: Characterizing cell department orientation in Fzd7-PDB, Fzd7 or Hycamtin kinase activity assay Vangl2 expressing tissue. elife-23279-fig3-figsupp2-data1.xlsx (53K) DOI:?10.7554/eLife.23279.021 Body 4source data 1: Quantitative characterization of clone morphology in Fzd7-PDB expressing tissue. elife-23279-fig4-data1.xlsx (9.0K) DOI:?10.7554/eLife.23279.031 Body 4source data 2: Distinguishing one Hycamtin kinase activity assay and multiple stacks in in Fzd7-PDB expressing tissue. elife-23279-fig4-data2.xlsx (8.6K) DOI:?10.7554/eLife.23279.032 Body 4source data 3: Stack orientation analysis in Fzd7-PDB expressing tissue. elife-23279-fig4-data3.xlsx (8.9K) DOI:?10.7554/eLife.23279.033 Body 4source data 4: Quantitative characterization of clone morphology in Fzd7 expressing tissue. elife-23279-fig4-data4.xlsx (39K) DOI:?10.7554/eLife.23279.034 Determine 5source data 1: Pearson correlation analysis of Fzd7 mutants and phalloidin colocalization. elife-23279-fig5-data1.xlsx (9.8K) DOI:?10.7554/eLife.23279.041 Physique 6source data 1: Fluorescence intensity measurement of endogenous junctional Ncad in wild-type tissues. elife-23279-fig6-data1.xlsx (14K) DOI:?10.7554/eLife.23279.050 Determine 6source data 2: Fluorescence intensity measurement of junctional Ncad-GFP in wild-type, Fzd7-PDB or Fzd7 expressing tissues. elife-23279-fig6-data2.xlsx (19K) DOI:?10.7554/eLife.23279.051 Physique 6source data 3: Quantitative analysis of sister cell contact in the tissues treated with Rabbit polyclonal to INPP4A -Ncad antibody. elife-23279-fig6-data3.xlsx (24K) DOI:?10.7554/eLife.23279.052 Physique 6source data 4: Quantitative analysis of sister cell contact in dnNcad expressing tissues. elife-23279-fig6-data4.xlsx (24K) DOI:?10.7554/eLife.23279.053 Determine 6figure supplement 1source data 1: Pearson correlation analysis of junctional Ncad and phalloidin signal in wild-type tissues. elife-23279-fig6-figsupp1-data1.xlsx (15K) DOI:?10.7554/eLife.23279.046 Determine 6figure supplement 3source data 1: Cell-cell contact analysis in Ncad-GFP expressing tissues. elife-23279-fig6-figsupp3-data1.xlsx (29K) DOI:?10.7554/eLife.23279.047 Determine 6figure supplement 3source data 2: Cell-cell contact analysis in Ncad-GFP and Fzd7-PDB expressing tissues. elife-23279-fig6-figsupp3-data2.xlsx (32K) DOI:?10.7554/eLife.23279.048 Determine 6figure supplement 3source data 3: Cell-cell contact analysis in Ncad-GFP and Fzd7 expressing tissues. elife-23279-fig6-figsupp3-data3.xlsx (33K) DOI:?10.7554/eLife.23279.049 Abstract Both oriented cell divisions and cell rearrangements are critical for proper embryogenesis and organogenesis. However, little is known about how these two cellular events are integrated. Here we examine the linkage between these processes in chick limb cartilage. By combining retroviral-based multicolor clonal analysis with live imaging, the results show that one chondrocyte precursors can generate both single-column and multi-column clones through focused division accompanied by cell rearrangements. Concentrating on one column development, we show that stereotypical tissue structures is established with a pivot-like procedure between sister cells. After mediolateral cell department, N-cadherin is certainly enriched in the post-cleavage furrow; one cell pivots across the various other after that, leading to stacking right into a column. Perturbation analyses demonstrate that planar cell polarity signaling allows cells to pivot in direction of limb elongation via this N-cadherin-mediated coupling. Our function provides brand-new insights in to the systems generating appropriate tissues structures of limb skeleton. solid class=”kwd-title” Analysis organism: Chicken Launch A Hycamtin kinase activity assay central issue in contemporary biology is certainly how cells create a complicated tissues within a four dimensional (xyz and t) framework. That is accurate in developing embryos especially, where cells undergo elaborate behaviors including proliferation, differentiation and migration, while getting together with similar aswell as specific cell types. Two fundamental mobile processes, focused cell cell and divisions rearrangements, play important jobs during tissue development (Morin and Bella?che, 2011; Hardin and Walck-Shannon, 2014). By orienting the axis of department within a stereotypic direction,.